Lime

نویسندگان

  • Naděžda Brdičková
  • Tomáš Brdička
  • Pavla Angelisová
  • Ondrej Horváth
  • Jiří Špička
  • Ivan Hilgert
  • Jan Pačes
  • Luca Simeoni
  • Stefanie Kliche
  • Camilla Merten
  • Burkhart Schraven
  • Václav Hořejší
چکیده

Lymphocyte membrane rafts contain molecules critical for immunoreceptor signaling. Here, we report identification of a new raft-associated adaptor protein LIME (Lck-interacting molecule) expressed predominantly in T lymphocytes. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation. Thus, LIME appears to be involved in regulation of T cell activation by coreceptors.

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عنوان ژورنال:
  • The Journal of Experimental Medicine

دوره 198  شماره 

صفحات  -

تاریخ انتشار 2003